The removal of viruses during the purification of equine antisera using filtration aids Hyflo Super-Cel and Fulmon Super A.
نویسندگان
چکیده
The manufacturing process in Australia for equine antisera against various venoms/toxins is based primarily on ammonium sulphate precipitation of pepsin-digested IgG, whereby Fc and F(ab')(2)fragments are separated. The capacity of the process to remove non-enveloped and enveloped model viruses was assessed using a scaled-down process. Each virus was added to mid-process samples from equine plasma before the material was applied to Hyflo Super-Celtrade mark filtration followed by Fulmonttrade mark Super A filtration. Samples were analysed pre- and post-filtration and the log clearance of the viruses calculated. The mean clearance factors for viral load of canine adenovirus type II (CAV(2)), poliovirus type 1 (PV1), infectious bovine rhinotracheitis virus (IBR) and canine distemper virus (CDV) were 5.3 logs, 4.2 logs, 5.7 logs and 4. 0 logs respectively. Clearance results as virus is adsorbed to the filtration aids which are removed from the process, thereby demonstrating improved viral safety of equine antisera produced by CSL.
منابع مشابه
Chromatography of Blood-clotting Factors and Serum Proteins on Columns of Diatomaceous Earth
1. In batch adsorptions with prothrombin solutions, hyflo was the weakest adsorbent, standard super-cel intermediate, and filter-cel strongest. Of these three grades of diatomaceous earth, hyflo has the smallest surface area per gram and filter-cel the largest. In parallel breakthrough experiments, a column of standard super-cel had a capacity almost six times that of a hyflo column. 2. After p...
متن کاملMore recently in 1954, Dorfman and coworkers3) submitted a crude polysaccharide mixture from rabbit skin to electrophoresis on a slab of Hyflo Super-Cel and found that an itinsulfuric acid preparation thus purified showed the carbazol reaction for hexuronic
متن کامل
An empirical study on statistical analysis and optimization of EDM process parameters for inconel 718 super alloy using D-optimal approach and genetic algorithm
Among the several non-conventional processes, electrical discharge machining (EDM) is the most widely and successfully applied for the machining of conductive parts. In this technique, the tool has no mechanical contact with the work piece and also the hardness of work piece has no effect on the machining pace. Hence, this technique could be employed to machine hard materials such as super allo...
متن کاملThe effect of nitrogen mustards on the viscosity of thymonucleate.
The nitrogen mustards used in these experiments were the hydrochlorides of ethyl bis (/3 chloroethyl) amine, methyl bis (/3 chloroethyl) amine, and tris (fl chloroethyI) amine. Sodium nucleate was prepared from calf thymus by a modification of Hammarsten's procedure (5). After the preliminary extraction, filtration, and addition of CaC12, a precipitate was obtained by bringing the final ethanol...
متن کاملSeasonal Outbreak of Influenza A virus Infection in Pediatric Age Groups During 2004-2005 in South of Iran
Background: The pandemic and regional influenza outbreaks resulting from antigenic variation of influenza viruses have been the subject of numerous studies which are crucial to the preparation of the vaccine. Frequent global winter outbreaks of influenza viruses require a constant surveillance of emerging influenza variants in order to develop efficient influenza vaccine. Methods: This study wa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Biologicals : journal of the International Association of Biological Standardization
دوره 28 3 شماره
صفحات -
تاریخ انتشار 2000